Abstract: the purpose of this study is the possibility of stinky tofu brine standardization production of a try. In combination with related literature and fastidious, test was made six different formula, using mixed culture method and solid phase extraction - temperament online method for determining microbial different fermentation and end of the difference of volatile components. Experimental results show that the growth of microorganisms in the brine from fast to slow and in fermentation saturated and there is a downturn in mature stage. Soya bean with the old brine formula of yeast by the initial < 10 CFU/mL growth to x1013 CFU/mL, lactic acid bacteria from 0 < 10 CFU/mL growth to x1013 CFU/mL, the bacteria by the initial 4 x104 CFU/mL surged to x1014 CFU/mL, and the different recipes of brine in the microbial growth rate is not the same, such as yeast sauerkraut water finally only 2 x105 CFKey words: stinky tofu brine, mixed bacteria method, gram staining, sensory evaluation, flavour compounds.