Secondary structure in RNA targets is a significant barrier to short DNA However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent 目标RNA的二级结构对短DNA探针来说是一个严重的障碍,可是,如果这些靶RNA是人工体外增殖的产物,并且在三磷酸核苷存在的环境下,改变RNA的二级结构,DNA可以进行转录,并且不会影响杂交产物的特性非专业人士瞎翻的,下班了,没空了