Under the new economic conditions, culture is the soul of the enterprise, is an important component of the enterprise's core competitiveness. In the current wave of globalization and the new economy, under the general background of China's entry into the WTO. Port industry and the survival of the external environment has undergone tremendous changes in the internal operations, we welcome a rare development opportunity, also facing fierce competition and tough challenges. China ranks third in the port city of Qingdao in Shandong Province in order to technology networks and the information industry as the main support. to the characteristics of the global economic integration to the survival and development of a new economic era. Construction of a new enterprise culture in keeping with this imperative. This paper analyzes the port city under the new economic conditions in production and business activities of the new features, and on this basis, Analysis of the port city of the new economy to the challenges of building and updating the corporate culture, and their own point of view and corresponding countermeasures. Finally on this basis, in line with the new economic characteristics of the building "will, in the" corporate culture at the core of a new three-dimensional model envisaged.