As people's attention to environmental safety, pesticide effect of beneficial to the environment organisms also attracted attention. Through the determination of zebrafish this mould amine its environment beneficial not target creature's acute toxicity and biological reagent to understand the enrichment coefficients of environment influence, for research into the elixir effects to the environment provides the basis. This paper adopts half a static method for the determination of its of zebrafish mould amine acute toxicity and biological enrichment coefficients. With N, N - DMF (DMF) for fertilizer, preparation of different concentrations of its mildew amine solution, determination of zebrafish acute toxicity. Biological enrichment sex trials set its mildew amine concentration of 0.25 and 2.50 mg/L, continuous exposure time for 192h, determination BCF192h enrichment coefficients (biological). The results showed that its mildew amine LC50 is 24.9 mg/L, belongs to the lower toxicity, respectively for BCF192h 10