Duck LiMo epidemic Riemerella anatipestifer coli (RA) in China, the serum type complex influenza epidemic duck LiMo coli serum type reports in the majority with type 1. In type 1 strain, high serum free for clinical diagnosis and classification has important significance. The experiment will strain type 1 duck LiMo epidemic strain on expanding coli bacteria and concentrated training after's adjuvants and incomplete adjuvants emulsion's preparation's adjuvants inactivated vaccine and oil emulsion's not completely adjuvants inactivated vaccine, used oil emulsion immune rabbit. Immune program is as follows: using the free two injection's fins completely adjuvants inactivated vaccine 1ml/oil emulsion, first from the only after 10d 20d and to ml/only six injection RA's not completely adjuvants inactivated vaccine oil emulsion were free, three free. Three from 10 days after the blood test for 1 to 0, SiMian continue in 31 days, 33 days, 35 days of rabbit ears and along the intravenous RA microbial ml/ml/only only, , ml/only. After SiMian 9 days to try for the high price of blood, serum by heart bleed separation. The laboratory test and proof of clinical application, duck LiMo epidemic coli type 1 high serum free with antibody titer high and high specificity.