要PDF的话找我，我正在帮你找，好辛苦，你也搞论文吗？Clonal multiplication of Lycoris aurea by tissue culture 无性繁殖黄花石蒜的组织培养 This article is not included in your organization's subscription. However, you may be able to access this article under your organization's agreement with Elsevier. Li-Chun Huanga and Din-Ming Liua aInstitute of Botany, Academia Sinica Nankang, Taipei, Taiwan Accepted 28 December 1988. Available online 14 October 2003. Abstract A method has been developed for clonal propagation of Lycoris by starting with explants composed of pairs of bulb-scale segments, each 4 × 5 mm, joined basally by a 1-mm strip of stem plate. They were induced to undergo organogenesis by placement in darkness on a medium supplemented with 30 mg l?1 each of NAA and BA for the first 3 months. The swollen explants were then transferred to a second medium with 3 mg l?1 NAA and 10 mg l?1 BA and given illumination for differentiation of adventitious buds and emergence of shoots. The shoots were multiplied by transferring groups of 3 or more to fresh medium at 4-week intervals. To obtain plants, individual shoots were rooted in another medium with 3 mg l?1 NAA. The method is projected to yield at least 30 000 plants per bulb the first year. Keywords: bulb-scale; flower; Lycoris aurea; micropropagation; shoot tip; spider lily
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