Shegan Tissue Culture Abstract Shegan as a medicinal plants, it has qingrejiedu, fall blood detumescence, gas expectoranting etc. In recent years, clinically used in the treatment of bronchitis, mumps, mastitis, and hepatic coma symptoms such as good effect. In the conventional breeding, shoot dry seeds breeding, mainly in the breeding way against radiation dry. Therefore, the application of tissue culture, the rapid reproduction method can solve shoot dry traditional asexual reproduction problems, in order to make seedling large-scale production of standardized, and commercialization. This experiment with different concentrations of different hormones DuiShe dry sterile buds proliferation and root and callus induction study of impact, the experimental results show that:1 and in the choice of cytokinins, 6 - DuiShe BA dry buds best effect, the proliferation of 6 - BA induce clumps and robust. JiDai training bud, the setting reflects the good shot, induced several concentration ratio of the highest dry buds proliferation concentration of: MS + 6 - mg. L - 1. multiples of proliferation, tube seedlings on nicely. But BA to mg. L - 1, buds drop rate and growth , the dry rooting culture, four olumes concentration ( and , , ). One month after inoculation statistical results found: when the concentration of olumes rooting rate, root best effect, the average number of rooting for , 2,4-d different concentrations of callus inductionMS with + 3% in basic medium sugar, adding different concentrations 2,4-d (0, , 1, 2). Ten days after inoculation observation results show: 2,4-d concentration of callus induction of the affected, 2,4-d concentration 2mg. L - 1 for the best effect, when rateKeywords: shoot dry tissue culture