表面抗原的检测论文

Biosensors in food, medicine, environmental testing and many other fields have a wide range of applications, in recent years, the development of increasingly sophisticated。One of many biosensor for immunization on clinical research, it can significantly shorten the testing time, and easy to operate and difficult to pollution。The main thesis of this study the clinical diagnosis of hepatitis B virus immunosensor preparation, the use of sparse matrix (-SH) can form a stable with the Au-S bond, fixed at first thiourea on gold electrode, and then the use of cross-linking agent glyoxal hepatitis B antibody (HBsAb) fixed on the electrode to form the monolayer, made for the detection of hepatitis B surface antigen (HbsAg) Immunosensor current。Through cyclic voltammetry to examine the electrochemical properties of electrodes. The sensor produced a simple, convenient operation, short response time, high sensitivity, can be used to detect hepatitis B surface antigen。Studies have shown that the sensor detection range of 5-200ug / L, detection limit is 0.1 ug / L。

Biosensors, which have evolved gradually in recent years, have a bright application prospect in various fields such as foods, medicine, environment monitoring, etc. Among those biosensors, the immunology sensors, which are widely applied in clinical research, are capable of reducing test time significantly, besides, they are easily handled and not vulnerable to contamination. This article mainly focused on the preparation of the immunosensors for the clinical diagnosis of HBV. Utilizing the stable Au-S bond formed by the Mercapto group and gold, thiourea was bonded to the gold electrode, in subsequent steps, glyoxal crosslinker was utilized to bone HBsAb to the electrode, forming a monolayer. Thus, an amperometric immunosensor was prepared for the detection of HbsAg and the electrochemical properties of the electrode were examined by the cyclic voltammetry method. The abovementioned sensor, which is of short response time and high sensitivity, is easy to prepare and operate for the detection of HbsAg. Further research proves that the sensor has a detection range of 5-200ug/L, and a detection limit of 0.1ug/L. PS：请仔细核对专业名词。。。

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