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干旱对植物的影响非常广泛而深刻,它的影响可以表现在生长发育的各个阶段,如种子萌发、营养生长和生殖生长,直到开花结实。同时影响各种生理代谢过程,如光合作用、呼吸代谢、水分和营养元素的吸收运转、各种酶的活性和有机物质的转化、运输、积累等。植物体对干旱胁迫作出的响应即有形态解剖结构上的,如叶片和根系的生长、排列和结构的适应,又有生理上的。柽柳属植物长期生活在气候恶劣,土壤脊薄的环境中,形成了其特有的适应环境的生理生态特性。柽柳植物对干旱胁迫在生理上的响应和适应性表现在主动积累脯氨酸、可溶性糖、无机离子等物质参与渗透调节,调节体内脱落酸、生长素、赤霉素内源激素变化,调整膜系统保护酶超氧化物歧化酶、过氧化物酶活性。 1 材料与方法 1.1 材料 试验材料为甘蒙柽柳(Tamariax austrmongolica Nakai)、多枝柽柳(T. ramosissima Ledeb)、中国柽柳(T. chinesis Lour)、短穗柽柳(T. Laxa Wild)、刚毛柽柳(T. hispida Wild)、密花柽柳(T. arceuthoides Bge)、甘肃柽柳(T. gansuensis H. Z. Zhang)和短毛柽柳(T. karelinii Bge)采于中国科学院吐鲁番沙漠植物园。采后扦插于新疆农业大学林学院实验地内。每种柽柳插穗数目一致,插穗发芽后,加强养护管理,长至三月龄时进行处理,采用不浇水自然干旱方式,处理60天,每隔3天采一样,采样时间为北京时间早上10点。测定距地面15-20cm处小枝,重复三次。1.2 方法 脯氨酸测定参照朱广镰方法;可溶性糖用蒽酮比色法测定;无机离子用浸提法测定;内源激素用HPLC法测定。 2 结论与分析 2.1 干旱胁迫对柽柳渗透调节物质的影响 渗透调节是植物在逆境条件下降低渗透势,抵抗逆境胁迫的一种重要方式。渗透调节为:在水分胁迫下,植物生长受到抑制,植物组织可以通过降低细胞的渗透势适应外界环境,这一现象称为渗透调节。目前已知,许多植物都具有渗透调节能力,不同植物及其品种渗透调节能力的大小不同;参于渗透调节的物质种类及作用不同。研究较多渗透调节物质主要有脯氨酸、可溶性糖、无机离子、甜菜碱等。干旱胁迫条件下,参与渗透调节物质分为两大类:一类是由外界进入植物细胞的无机离子;一类是在细胞内合成的有机溶质,在有机溶质中能理想地起渗透调节作用的化合物范围是很窄的,主要是多元醇和偶极含氮化和物。作为渗透调节的物质必须具备如下特征:分子量小、容易溶解;在生理PH范围内不带净电荷;必须能被细胞膜保持住;引起酶结构的变化的作用极小;在酶结构稍有变化时,能使酶构象稳定而不致溶解;生成迅速,并能累积到足以引起调节渗透势的作用量。2.1.1 脯氨酸干旱胁迫条件下柽柳植物体内脯氨酸含量显著增加,干旱处理初期,脯氨酸积累速率增加平缓,中后期脯氨酸积累速率达最大值。脯氨酸积累对植物抗旱有益。由于脯氨酸是水溶性最大的氨基酸(162.3克/百克水,25℃)易于水合或具有较强的水合力,植物受到水分胁迫时它的增加有助于细胞或组织的持水,防止脱水,这有助于提高其抗旱性。且脯氨酸对柽柳植物无毒害作用,能参与叶绿素的合成和对蛋白质起到保护作用。伴随着干旱胁迫的发展,脯氨酸生物合成来源谷氨酸由于碳水化合物供应受阻,将影响其合成,进而影响脯氨酸的合成,因此脯氨酸的积累最终也会下降和停止。柽柳主动积累脯氨酸参与渗透调节,这是其适应干旱环境的生理基础之一。2.1.2 无机离子水分胁迫时植物可以通过累积细胞内无机离子作为渗透调节物质,累积无机离子的量、种类,因植物、品种和器官的不同而有差异。干旱胁迫条件下柽柳植物主动积累k+、Ca2+、Na┼;主动积累k+有利于柽柳在干旱胁迫条件下关闭气孔,减少水分损失;有利于脯氨酸积累;有利于酶活性的保持;从而维持柽柳正常的细胞功能。主动积累Ca2+有利于柽柳通过钙第二信使的作用利用钙调素的中介而把胞外信号转换成胞内的生理生化反应;钙也是柽柳必需的大量营养元素。柽柳大量积累Na┼可能有两个原因,一是Na┼主动积累降低柽柳细胞渗透势,参与渗透调节;二是干旱胁迫使得柽柳泌盐腺被破坏,泌盐功能不能正常发挥,使得柽柳他体内吸收的多余Na┼不能及时排除体外,在细胞内积累。植物对无机离子的吸收是一主动过程,细胞中无机离子浓度可大大超过外界介质中的浓度。2.1.3 可溶性糖 干旱胁迫条件下柽柳组织的可溶性糖含量明显增加,作为渗透调节物质的可溶性糖主要有蔗糖、葡萄糖、果糖、半乳糖等;逆境下柽柳植物体内可溶性糖增加的原因可能有,大分子碳水化合物和蛋白质的分解加强而合成受到抑制,蔗糖的合成则加快光合产物形成过程中直接转向低分子量的物质蔗糖等,而不是淀粉;从植物体其他部分输入有机溶质糖。柽柳主动积累可溶性糖参与降低其体内渗透势,以利于其在干旱生境下维持植物体正常生长所需水分。 整个干旱处理过程,柽柳植物脯氨酸与可溶性糖积累进程不同,脯氨酸在干旱后期含量降低,可溶性糖在干旱后期大量增加。对柽柳植物渗透调节而言,严重干旱会使其渗透调节能力丧失。因此,可溶性糖在干旱后期大量积累可能是脯氨酸下降的补偿策略。2.2 干旱胁迫对柽柳内源的激素的影响 2.2.1 脱落酸(ABA)干旱胁迫条件下柽柳体内脱落酸含量显著增加,随着胁迫程度的加剧,脱落酸含量迅速大量增加。干旱引起柽柳内源脱落酸迅速累积,表明柽柳对干旱胁迫做出了反应,为适应干旱生境,脱落酸调节气孔,使之关闭,尽量减少不必要水分消耗,增强植物保水能力;它还能促使脯氨酸加速积累,增强植物体的渗透调节能力。2.2.2 吲哚乙酸(IAA)干旱胁迫条件下,柽柳内源IAA明显增加。有关水分胁迫对内源吲哚乙酸水平的效应的研究较少而且结论不一致。植物体内可扩散IAA含量一般随土壤含水量的降低而降低,但是有报道说巢菜叶内的IAA含量在一个干旱周期内却增加了,和乙烯及ABA水平的变化相比,其变化较缓慢。许多研究表明,尚不能确定地说,随着干旱胁迫的加剧IAA含量增多。迄今在水分状况和内源IAA水平之间的特定关系方面,尚不能做出定性结论,其内在机制尚需深入研究。2.2.3 赤霉素(GA)干旱胁迫条件下,柽柳体内GA在干旱处理前期增加不显著,干旱处理后期大量增加。GA对 IAA具有调节作用,表现在(1)GA抑制IAA氧化酶及过氧化物酶的活性,使生长素含量增加。(2)GA促进束缚型IAA释放出自由型IAA,从而提高了IAA含量。柽柳GA、 IAA都增加表明,干旱胁迫下GA含量增加,相应也促进IAA含量的增加。2.3 干旱胁迫对柽柳膜系统保护酶的影响 2.3.1 超氧化物歧化酶(SOD)超氧化物歧化酶是膜脂过氧化防御系统的主要保护酶,它催化活性氧发生歧化反应,产生无毒分子氧和水,从而避免植物遭受伤害,较高的SOD活性是植物抵抗逆境胁迫的生理基础。干旱胁迫条件下,柽柳SOD活性在干旱处理前期增高后期降低。2.3.2 过氧化物酶(POD)过氧化物酶(POD)可除去干旱胁迫条件下植物体生理系统中累积的H2O2。干旱胁迫条件下,柽柳POD酶活性的变化趋势与SOD的一致,即先上升而后下降。但POD活性升至最高的时间早,这表明柽柳的POD比SOD对干旱更为敏感,干旱处理后期,SOD仍有较高活性,POD则在干旱后期活性明显降低。2.3.3 丙二醛(MDA)MDA是膜脂过氧化的主要产物之一,是有细胞毒性的物质,能够引起细胞膜功能紊乱。且对许多功能分子有破坏作用,因此MDA含量增加是植物细胞损伤的直接原因。干旱胁迫条件下,柽柳MDA含量增加。干旱前期MDA缓慢增加,而后急剧增加。这表明干旱初期,胁迫程度较轻,SOD、POD活性升高,柽柳防御系统发挥作用,能抵御干旱造成的伤害,但后期随着胁迫程度的加剧,膜系统保护酶SOD、POD活性下降,防御系统作用减小,MDA含量显著增加。柽柳MDA含量随着胁迫程度的增加而增加,说明干旱促进了膜脂过氧化作用。--------------------------------------------------------------------------------作者简介: 王霞 女 (1968.1—) 讲师 硕士 现工作于新疆农业大学林学院 邮编 830052 主要从事干旱区植物抗旱性生理的研究 参加中科院知识创新项目“塔里木河下游绿色走廊保护于土地荒漠化防治研究示范”和国家自然基金资助项目关于”柽柳生物多样性研究”(39470085)两项课题 发表5篇论文 联系电话 4523001—2382(办) 4536208(宅)
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2012年1)Ding Wenjuan,Wang Renqing, Yuan Yifu, Liang Xiaoqin, Liu Jian. 2012.Effects of nitrogen deposition on growth and relationship ofRobinia pseudoacaciaandQuercus acutissimaseedlings. Dendrobiology, 67: 3-13.2)Zhang Juan, Dai Jiulan, Du Xiaoming, Li Fasheng, Wang Wenxing,Wang Renqing. 2012. Distribution and sources of petroleum-hydrocarbon in soil profiles in Hunpu wastewater-irrigated area, China’s northeast. Geoderma, 173: 215-2233)Zhang Juan, Dai Jiulan, Haorui Chen, Du Xiaoming, Wang Wenxing, Wang Ning,Wang Renqing. 2012. Petroleum contamination in groundwater/air and their effects on farmland soil at the outskirt of an industrial city, China. Journal of Geochemical Exploration.In press(DOI: 10.1016/j.gexplo.2012.04.002)4)Wang Qiang,Wang Renqing, Tian Chengyu, Yu Yue, Zhang Yongli, Dai Jiulan. 2012. Using microbial community behavior as the complementary environmental condition indicator: a case study of an iron deposit tailing area. European Journal of Soil Biology, 51: 22-29.5)Chen Hua,Wang Renqing, Ge Xiuli, Zhang Juan, Du Ning, Wang Wei, Liu Jian. 2012. Competition and soil fungi affect the physiological and growth traits of an alien and a native tree species. Photosynthetica, 50(1): 77-85.6)Wang Shujun, Liu Jian,Wang Renqing, Ni Zirong, Xu Shipeng, Sun Yueyao. 2012. Impact of socio-economic development on ecosystem services and its conservation strategies: a case study of Shandong Province, China. Environmental Monitoring and Assessment, 184(5): 3213-3229.7)Chen Hua,Liu Jian, Xue Tong,Wang Renqing. 2012. Roads accelerate the invasion process of alien species. Advanced Materials Research, 347-353: 1483-1487.8)Yu Yue, Wang Hui, Liu Jian, Wang Qiang, Shen Tianlin, Guo Weihua,Wang RenQing. 2012. Shifts in microbial community function and structure along the successional gradient of coastal wetlands in Yellow River Estuary. European Journal of Soil Biology,49:12-21.9)Ge Xiuli,Liu Jian,Wang Renqing.2012. The comparison between the historical and current vegetation in Nansi Lake area. Advanced Materials Research, 518-523: 5180-5184.10)Xue Tong, Dai Jiulan, Zhang Mengmeng,Wang Renqing. 2012. FT-IR spectroscopic structural characterization of forest soil and its adsorption and desorption for mercury. Pedosphere. Accepted.11)Zhang Mengmeng, Liu Jia, Tian Chengyu,Wang Renqing, Dai Jiulan. 2012. Effect of pH, temperature and the role of ionic strength on the adsorption of mercury (II) by typical Chinese soils. Communications in Soil Science and Plant Analysis, 43: 1599-1613.12)薛童,陈华,张依然,王仁卿,戴九兰. 2012.腐殖质化学的光谱研究新进展.安徽农业科学, 6: 3833-3836.13)郭微,戴九兰,王仁卿. 2012.溶解性有机质影响土壤吸附重金属的研究进展.土壤通报, 43(3):761-768.2011年14)Ding Wenjuan, Liu Jian, Wu Daqian, Wang Yue, Chang Cheinchi,Wang Renqing. 2011. Salinity stress modulates habitat selection in the clonal plantAeluropus sinensissubjected to crude oil deposition. The Journal of the Torrey Botanical Society, 183(3): 262-271.15)Wang Yutao, Liu Jian, Hansson Lars, Zhang Kai,Wang Renqing.2011. Implementing a relative stricter discharge standard in a regional industry to enhance eco-efficiency and sustainability: a case study of Shandong pulp and paper industry, China. Journal of Cleaner Production, 19(4): 303-310.16)Liu Lei, Liu Jian, Wang Yutao, Wang Nvjie,Wang Renqing.2011. Cost-benefit analysis and ecological compensation of watershed-scale wetland restoration: a case study in Shandong province,China.International Journal of Environmental Research, 5(3): 787-796.17)Chen Hua, Liu Jian, Zhang Yongli, Wang Qiang, Ge Xiuli, Wei Yinghua,Wang Renqing. 2011. Influence of invasive plantCoreopsis grandifloraon functional diversity of soil microbial communities. Journal of Environmental Biology, 32(5): 567-572.18)王仁卿,杜宁,郭卫华,张淑萍,戴九兰,刘建,王玉志,王玉涛.2011.走向国际化的生态学——山东大学生态学的过去、现在和将来.山东大学学报(理学版), 46(10):197-207.19)张孟孟,戴九兰,王仁卿. 2011.溶解性有机质对土壤中汞吸附迁移及生物有效性的影响研究进展.环境污染与防治, 5: 95-99, 110.20)Han Xuemei,Wang Renqing, Guo Weihua, Pang Xugui, Zhou Juan, Wang Qiang, Zhan Jincheng, Dai Jierui. 2011. Soil microbial community response to land use and various soil elements in a city landscape of North China. African Journal of Biotechnology, 10(73): 16554-16565.21)Guo Weihua, Jeong Jihee, Kim Zinsuh,Wang Renqing, Kim Enhye, Kim Sunghwan. 2011. Genetic diversity ofLilium tsingtauensein China and Korea revealed by ISSR markers and morphological characters. Biochemical Systematics and Ecology, 39: 352–360.22)Guo Weihua, Li Bo, Zhang Xinshi,Wang Renqing. 2011. Water balance in SPAC under water stress: a case study of Hippophae rhamnoides and Caragana intermedia. In: Efe R. (Eds). Environment and Ecology in the Mediterranean Region. Cambridge Scholars Publishing.23)Wang Wei, Wang Renqing, Yuan Yifu, Du Ning, Guo Weihua.2011. Effects of salt and water stress on plant biomass and photosynthetic characteristics of Tamarisk (Tamarix chinensisLour.) seedlings. African Journal of Biotechnology, 10(78): 17981-17989.24)Zhou Juan,Wang Renqing, Guo Weihua, Zhou Guangjun, Wang Qiang, Wang Wei, Han Xuemei, Pang Xugui, Zhan Jincheng, Dai Jierui. 2011. Soil microbial community diversity and its relationships with geochemical elements under different farmlands in Shouguang, China. Communications in Soil Science and Plant Analysis, 42: 1008-1026.25)杜宁,张秀茹,王炜,陈华,谭向峰,王仁卿,郭卫华. 2011.荆条叶性状对野外不同光环境的表型可塑性研究.生态学报, 31(20): 6049-6059.2010年26)Liu Jia, Dai Jiulan,Wang Renqing, Li Fasheng, Du Xiaoming, Wang Wenxing. 2010. Adsorption/desorption and fate of mercury (II) by typical black soil and red soil in China. Soil and Sediment Contamination, 19: 587-601.27)Wang Qiang, Dai Jiulan, Yu Yue, Zhang Yongli, Shen Tianlin, Liu Jiangsheng,Wang Renqing. 2010. The changes of soil microbial structural and functional characterizations under the stress of heavy metals in typical agriculture soil. Environmental Monitoring and Assessment. 161: 495-508.28)Du N., Guo W. H., Zhang X. R.,Wang R. Q. 2010. Morphological and physiological responses ofVitex negundoL. var.heterophylla(Franch.) Rehd. to drought stress. Acta Physiol Plant, 32: 839-848.29)Guo W. H., Li B., Zhang X. S.,Wang R. Q. 2010. Effects of water stress on water use efficiency and water balance components ofHippophae rhamnoidesandCaragana intermediain the soil–plant–atmosphere continuum. Agroforestry Systems, 80: 423–435.30)Wu D. Q., Liu J., Wang S. J.,Wang R. Q.2010. Simulating urban expansion by coupling a stochastic cellular automata model and socioeconomic indicators. Stoch Environ Res Risk Assess, 24: 235–245.31)Wu Daqian, Liu Jian, Wang Shujun,Wang Renqing.2010. Simulating urban expansion by coupling a stochastic cellular automata model and socioeconomic indicators. Stochastic Environmental Research and Risk Assessment, 24(2): 235-245.32)徐飞,郭卫华,徐伟红,王仁卿.2010.刺槐幼苗形态、生物量分配和光合特性对水分胁迫的响应.北京林业大学学报, 32 (1): 24-30.33)吴大千,王仁卿,高甡,丁文娟,王炜,葛秀丽,刘建. 2010.黄河三角洲农业用地动态变化模拟与情景分析.农业工程学报, 26(4): 285-290.34)葛秀丽,刘建,王镥权,李卫东,王仁卿.2010.南四湖区域乡土植物生态特性研究.见:第十三届世界湖泊大会论文集(The 13th World Lake Conference).北京:中国农业大学出版社,pp. 2725-2728.35)王强,戴九兰,吴大千,余悦,申天琳,王仁卿. 2010.微生物生态研究中基于BIOLOG方法的数据分析.生态学报, 30(3): 817-823.36)何欢,申天琳,戴九兰,郭微,王强,马丽,王仁卿. 2010.潮土潜在硝化速率对重金属锌镉的响应研究.农业环境科学学报, 29(5): 918-922.37)王强,戴九兰,付合才,申天琳,吴大千,王仁卿. 2010.空间分析方法在微生物生态学研究中的应用.生态学报, 30(2): 439-446.38)王女杰,刘建,吴大千,高甡,王仁卿.2010.基于生态系统服务功能的区域生态补偿研究——以山东省为例.生态学报,30(23): 6646-6653.2009年39)Dai J. L., Zhang M., Hu Q. H., Huang Y. Z.,Wang R.Q., Zhu Y. G. 2009. Adsorption and desorption of iodine by various Chinese soils: II. Iodide and iodate. Geoderma, 153: 130-135.40)Wang S. J., Li Jennifer, Wu D. Q., Zhang K.,Wang R.Q., Liu L.2009. The ecological impact assessment of urban development policies: a case study of Ji’nan city, China. Journal of Environmental Assessment Policy and Management, 4: 427-450.41)Wang S. J., Liu J., Ren L. J., Zhang K.,Wang R.Q.2009. The development and practices of Strategic Environmental Assessment in Shandong Province, China. Environmental Impact Assessment Review, 29: 408-420.42)Wang S. J., Jennifer Li, Wu D. Q. , Liu J., Zhang K.,Wang R.Q.2009. The Strategic Ecological Impact Assessment of urban development policies: a case study of Rizhao City, China.. Stochastic Environmental Research and Risk Assessment, 23: 1169-1180.43)Wu D. Q. , Liu J., Zhang G. S., Ding W. J., Wang W.,Wang R.Q.2009. Incorporating spatial autocorrelation into cellular automata model: an application to the dynamics of Chinese tamarisk (Tamarix chinensis Lour.). Ecological Modelling, 220: 3490-3498.44)Xu F., Guo W. H., Xu W. H., Wei Y. H.,Wang R.Q.2009. Leaf morphology correlates with water and light availability: What consequences for simple and compound leaves. Progress in Natural Science, 19: 1789 -1798.45)Xu F., Guo W. H.,Wang R.Q., Xu W. H., Du N., Wang Y. F. 2009. Leaf movement and photosynthetic plasticity of black locust(Robinia pseudoacacia)allebiate stress under different light and water conditions. Acta Physiologiae Plantarum, 31: 553 - 563.46)Yang J. H., Zhang S. P., Liu J., Zhai W.,Wang R.Q.2009. Genetic diversity of the endangered species Rosa rugosa Thunb. in China and implications for conservation strategies. Journal of Systematics and Evolution,47(6): 515-524.47)Zhang J., Dai J. L.,Wang R.Q., Li S. F., Wang W. X. 2009. Adsorption and desorption of divalent mercury (Hg2+) on humic acids and fulvic acids extracted from typical soils in China. Colloids and Surfaces A: Physicochemical and Engineering Aspects,335: 194-201.48)王玉涛,郭卫华,刘建,王淑军,王琦,王仁卿2009.昆嵛山自然保护区生态系统服务功能价值评估.生态学报,9 (1): 523-531.49)吴大千,刘建,贺同利,王淑军,王仁卿. 2009.基于土地利用变化的黄河三角洲生态服务价值损益分析.农业工程学报, 2009, 25 (8): 256-261.50)吴大千,刘建,王炜,丁文娟,王仁卿. 2009.黄河三角洲植被指数与地形要素的多尺度分析.植物生态学报, 33 (2):237-245.2008年51)Dai J. L., Li S. J., Zhang Y. L.,Wang R.Q. 2008. Distributions, sources and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in topsoil at Ji’nan city, China. Environmental Monitoring and Assessment, 147: 317-326.52)Liang Y., Liu J., Zhang S. P., Wang S. J., Guo W. H. andWang R.Q. 2008. Genetic diversity of the invasive plant Coreopsis grandiflora at different altitudes in Laoshan Mountain, China. Canadian Journal of Plant Science, 88(4):831-83753)Liu J., He W. M., Zhang S. M., Liu F. H., Dong M.,Wang R.Q.2008. Effects of clonal integration on photosynthesis of the invasive clonal plant Alternanthera philoxeroides.Photosynthetica,46(2): 299-302.54)Zhang Y. L., Dai J. L.,Wang R.Q., Zhang J. 2008. Effects of long-term sewage irrigation on agricultural soil microbial structural and functional characterizations in Shandong, China. European Journal of Soil Biology, 44(1): 84-91.55)Zhou J., Guo W. H.,Wang R.Q., Han X. M., Wang Q. 2008. Microbial community diversity in the profile of an agricultural soil in northern China. Journal of Environmental Sciences, 20: 981-988.56)刘江生,王仁卿,张永利,王强,戴九兰. 2008.山东省黄河故道土壤环境背景值研究.环境科学, 29(6):1699-1704.57)徐飞,郭卫华,徐伟红,王仁卿. 2008.短期干旱和复水对麻栎幼苗光合及叶绿素荧光的影响.山东林业科技, 4: 1-5.58)张高生,王仁卿. 2008.现代黄河三角洲生态环境的动态监测.中国环境科学, 28(4): 380-384.59)张高生,王仁卿. 2008.现代黄河三角洲植物群落数量分类研究.北京林业大学学报, 30:31-36.2007年60)Guo W. H., Liu H., Du N., Zhang X. S.,Wang R. Q. 2007. Structure design and establishment of database application system for alien species in Shandong Province. Journal of Forestry Research, 18 (10): 11-16.61)Guo W. H., Li B., Zhang X. S.,Wang R. Q.2007. Architectural plasticity and growth responses of Hippophae rhamnoides and Caragana intermedia seedlings to simulated water stress. Journal of Arid Environments, 69(3): 385-399.62)Han X. M., Guo W. H.,Wang R. Q., Wang M. C. 2007. Microbial communities in microcosm soils treated with battery waste. 17(9): 29-36.63)Han X. M., Guo W. H.,Wang R. Q., Zhou J., Wang M. C.2007. Effects of vegetation type on soil microbial community structure and catabolic diversity in five plant communities in North China assessed by polyphasic methods. Journal of Environmental Sciences, 19(10): 1228-1234.64)Liu C. C.,Wang R. Q.2007. Leaflet movement of Robinia pseudoacacia in response to a Changing light environment. Journal of integrative plant biology, 49(4): 419-424.65)Zhang G. S.,Wang R. Q., BaiminSong. 2007. Plant Community Succession in Modern Yellow River Delta, China. Journal of Zhejiang University Science, 8(8): 540-548.66)Zong M.J., Han X.M., Zhang Z.G.,Wang R.Q.2007. DNA recovery from soil in restoration area and indicated biodiversity. Ekológia (Bratislava), 26: 381-389.67)杜宁,郭卫华,王仁卿. 2007.昆嵛山典型植物群落生态学特征研究.生态学杂志, 26(2): 151-15868)杜宁,郭卫华,王仁卿. 2007.嵛山典型林下灌草层植物种间关系研究.山东大学学报, 42(3): 71-8369)郭卫华,李波,张新时,王仁卿. 2007.水分胁迫对沙棘(Hippophae rhamnoides)和中间锦鸡儿(Caragana intermedia)蒸腾作用影响的比较.生态学报, 27 (10): 4132-4140.70)王瑶,刘建,王仁卿. 2007.阿利效应及其对生物入侵和自然保护中小种群管理的启示.山东大学学报, 42 (1): 76-82.71)徐飞,郭卫华,王炜,徐伟红,王玉芳,王仁卿. 2007.黄河三角洲柽柳与芦苇光合特性比较.山东林业科技, 6: 29-33.72)徐飞,郭卫华,王玉芳,王炜,杜宁,王仁卿. 2007.济南市校园6个绿化树种光合荧光特征比较初探.山东大学学报,42 (5): 86-94.73)吴大千,杜宁,王炜,翟雯,王玉芳,王仁卿,张治国. 2007.昆嵛山森林群落下灌草层结构与多样性研究.山东大学学报, 42(1): 1-7.74)吴大千,徐飞,郭卫华,王仁卿,张治国. 2007.中国北方城市常见绿化植物夏季气孔导度影响因素及模型比较.生态学报, 10: 4141-4148.75)徐伟红,郭卫华,徐飞,王仁卿. 2007.三种枣树叶绿素荧光参数的日变化.山东农业科学, 2: 29-32.76)吴彤,李俊祥,戴洁,王仁卿. 2007.山东省外来植物的区系特征及空间分布.生态学杂志, 26 (4): 489-494.77)张永利,戴九兰,王仁卿,王文兴. 2007.土壤重金属污染的微生物生态效应研究进展.生态科学, 26 (2): 186-190.2006年78)Zhang X. Q., Liu J., Clive V. J. Welham, Liu C. C., Li D. N., Chen L.,Wang R. Q. 2006. The effects of clonal integration on morphological plasticity and placement of daughter ramets in black locust (Robinia pseudoacacia). Flora, 201(7): 547-55479)韩雪梅,郭卫华,周娟,张淑萍,王仁卿.2006.土壤微生物生态学研究中的非培养方法.生态科学,25(1): 87-9080)王嘉,王仁卿,郭卫华. 2006.重金属对土壤微生物影响的研究进展.山东农业科学, 1: 101-105.81)张宪强,郭卫华,杨继红,刘成程,王仁卿. 2006.刺槐(Robinia pseudoacacia)无性系种群结构与生长动态的研究.山东大学学报, 41 (2): 135-139.82)周娟,郭卫华,宗美娟,韩雪梅,王仁卿. 2006.房干村不同植被下可培养细菌多样性研究.山东大学学报, 41 (6): 161-167.83)宋楠,宋亚囝,王仁卿,郭卫华,刘建. 2006.山东省农业外来植物入侵现状及防治对策分析.山东科学, 19 (3): 15-21.2005年84)Liu J., Liang S. C., Liu F. H.,Wang R. Q., Dong M. 2005. Invasive alien plant species in China: regional distribution patterns. Diversity and Distribution, 11(4): 341-347.85)Liu J., Zhu X. W.,Wang R. Q., Dong M. 2005. Effects of grasshoppers on the dominant plants naturally growing in degraded grassland ecosystem in Northern China. Ekologia (Bratislava), 24 (2): 117-124.86)Qi X. S., Zhang S. P., Wang Y. Z.,Wang R. Q.,. 2005. Advantages of the integrated pig-biogas-vegetable greenhouse system in North China. Ecological Engineering, 24(3): 177-185.87)刘建,刘凤红,董鸣,王仁卿. 2005.浑善达克沙地南缘榆树种群的大小结构和邻体格局.中国沙漠, 25 (1): 75~80.88)鲁敏,李英杰,王仁卿. 2005.油松容器育苗基质性质与苗木生长及生理特性关系.林业科学, 41 (4): 86~93. .89)齐鑫山,丁卫建,王仁卿,聂岩,田明英. 2005.果园间种白三叶草对土壤生态及果树生产的影响.农村生态环境. 21(2): 13~1790)宋志文,王仁卿,席俊秀,韩潇源,孙贤风,王玮. 2005.荣成人工湿地净化效果的季节和年际变化.农村生态环境, 21 (4): 43~48.91)宋志文,王仁卿,席俊秀,韩潇源,姚晨. 2005.人工湿地对氮、磷的去除效率与动态特征.生态学杂志, 24(6) : 648-65192)张淑萍,王仁卿,杨继红,张强,魏英华. 2005.胶东海岸野生玫瑰(Rosa rugosa)的濒危现状与保护策略.山东大学学报, 40 (1): 112~118.93)张永利,张宪强,王仁卿. 2005. 鲁中山区植物区系初步研究.山东林业科技, 1: 1~5.
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