抗结核药物研究论文

Abs tract: The antimycobacterial activities of Osthole and 8-MOP against Mycobacterium tuberculosis H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were determined by the microdilution MABA Assay (MABA) and the conventional two folds dilutions method for screening candidate herbs. The cytotoxicity was determined by MTT method in splenic cell and hepatic cells of BALB/c mouse. Osthole showed a minimal inhibitory concentration (MIC) of 32 μg/mL against H37Rv and H37Ra, and 8-MOP showed a MIC of 128 μg/mL against M. tuberculosis H37Rv and and 8-MOP were no cytotoxicity to the cells. The results would pave the way to further development and exploring the mechanism of Osthole and 8-MOP against Mycobacterium.

Abstract: tuberculosis (TB) is one of the world's oldest and most widespread of a transmission through the respiratory tract of chronic infectious disease, which is caused by mycobacterium tuberculosis a beast cancer, chronic wasting diseases by everybody. With the development of aquaculture, frequent contact with animals such as cattle, deer, lead to the interaction of n/med tuberculosis bacterium infection. For decades, people in n/med tuberculosis bacterium has developed nearly 20 kinds of drugs, but there are side effects, especially on the side effects ofkidney is larger. At present, from the Chinese herbal medicine to find effective anti-tuberculosis components has become a hot interest and research. Anti-tuberculosis Chinese herbal medicine for screening of development value, through the son lahm LanWei plate method (Microdilution Alamar Blue Assay, MABA) and test tube method determination of oleanolic acid respectively for mycobacterium tuberculosis H37Rv (ATCC 27294) and H37Ra (ATCC 25177) in vitro antibacterial activity, namely the minimum bacteriostatic concentration (MIC), and compare the test results of two methods at the same time. Research results show that the oleanolic acid minimal inhibitory concentrations (mic) of these two kinds of n/med tuberculosis bacterium respectively are 16 (including g/mL; MABA method and test tube method comparison, entirely coincidence rate was 90% (9/10), has a good consistency. In this study for the development of oleanolic acid as the anti-tb drugs and its antibacterial mechanism research laid a solid foundation

已在国内务种期刊上发表或在全国学术会议交流论文100余篇，例如：《非高渗透压培养基培养细菌L型的研究》，王和，微生物学通报，1993，20（2）：101；《用非高渗培养基从慢性胆囊炎胆囊分离细菌L型》，王和等，中华医学检验杂志，1994，17（5）：263；《细菌在不同渗透压培养基上的胃霉素敏感性》，王和等，微生物学通报，1996，23（3）：161；《细胞壁缺陷细菌生物氧化特性的观察》，王和等，微生物学报，1998，38（5）：396；《慢性前列腺炎患者前列腺抗菌药物活性的检测》，王和等，中国微生态学杂志，2000，12（l）：76；《抗结核药物诱导结核分支杆菌形成L型及其特性的观察》，中华结核和呼吸杂志，2001，24（l）。 参编或主编著作三部，独著《男性生殖系统感染症的治疗》。主持的《细菌L型的非高渗培养和非高渗培养的细菌L型研究》项目获贵州省1994年度科技进步成果二等奖和首届青年科技大会优秀论文一等奖、《支原体无血清培养基的研制》获贵州省1996年度科技进步成果四等奖、《细胞壁缺陷细菌死亡的分子与生化机制》获员州省1998年度科技进步成果三等奖。