我在饲养动物的时候每组用了10只大鼠,但是在进行粪便16s和代谢组检测的时候,每组只送了6个平行样(再多平行样真是太贵了,测不起了),所以检测结果只有6个数据点。被审稿人发现了这个问题,想请教有动物实验经验的大神们,该如何回答这个问题。
“A total of 10 rats are included in each treatment group, but for some data (e.g. metabolomics, 16s RNA sequencing data) it is evident that only results from 6 rats are presented. This has to be clarified. Sampling description states sampling from all rats (line 133), so why are metabolomics data only from 6 rats?”