医学文献英文PPT

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This study investigated the effects of Na2SO3on the fat metabolism in human normal diploid HL-7702 (referred as L-02) After 24 h and 48 h, treatment with different concentrations ofNa2SO3, the intra and extra-hepatocellular triglyceride (TG) contents of L-02 were determined usingchemical-enzymatic The contents of very low-density lipoprotein (VLDL) and apolipoproteinB100 (apoB100) in the culture supernatants were determined using enzyme-linked immunosorbentassay (ELISA) Western blot was applied to detect the expressions of fatty acid oxidation and fat synthesisrelated proteins, VLDL assembly and secretion in L-02 本研究在人正常二倍体HL-7702的na2so3on脂肪代谢的影响（称为肝细胞L-02）。后24 h和48 h，用不同浓度ofna2so3处理，内、外肝细胞甘油三酯（TG）含量测定采用化学酶法对L-02。极低密度脂蛋白的含量（VLDL）和apolipoproteinb100（B100）培养上清中均采用酶联免疫吸附试验（ELISA）测定。Western blot检测脂肪酸氧化和脂肪synthesisrelated蛋白的表达，在L-02细胞VLDL的合成与分泌。